PROTECTIVE ANTIGENS OF EQUINE HERPESVIRUS TYPE-1
- 1 January 1978
- journal article
- research article
- Vol. 42 (2), 219-226
Abstract
Equine herpesvirus type 1 was cultivated in swine testis cell cultures and partially purified by differential centrifugation and centrifugation in a linear sucrose density gradient. The viral envelope was separated from the nucleocapsid by treatment with Rexol 25J followed by sucrose gradient centrifugation. The envelope and nucleocapsid preparations were then electrophoresed in polyacrylamide gel after solubilization with sodium dodecyl sulfate. Hamsters were immunized with various preparations of the partially purified virus, including live or inactivated equine herpesvirus type 1 and viral envelope and nucleocapsid, all derived from the Kentucky D strain of the virus. Challenge of the immunized hamsters, with a hamster-adapted strain of equine herpesvirus type 1 demonstrated protection only in those animals which were vaccinated with envelope-containing materials. When vaccination was carried out with fractions of electrophoresed envelope or nucleocapsid, protection was induced only by polypeptides of high MW containing a glycoprotein component of the envelope of equine herpesvirus type 1.This publication has 22 references indexed in Scilit:
- Purification of Equine Herpesvirus Type 1Journal of General Virology, 1976
- Structural polypeptides of the hamster strain of equine herpes virus type 1: Products associated with purificationVirology, 1974
- Studies of the molecular anatomy of the L-M cell strain of equine herpes virus type 1: Proteins of the nucleocapsid and intact virionVirology, 1974
- Herpes Simplex Virus-specific Polypeptides Studied by Polyacrylamide Gel Electrophoresis of Immune PrecipitatesJournal of General Virology, 1974
- A high resolution PAS stain for polyacrylamide gel electrophoresisAnalytical Biochemistry, 1973
- Equine abortion (herpes) virus: Purification and concentration of enveloped and deenveloped virus and envelope material by density gradient centrifugation in colloidal silicaVirology, 1972
- The proteins of enveloped and deenveloped equine abortion (herpes) virus and the separated envelopeVirology, 1971
- Proteins of the Murine C-Type RNA Tumour Viruses: Isolation of a Group-specific Antigen by Isoelectric FocusingJournal of General Virology, 1970
- Measles virusArchiv für die gesamte Virusforschung, 1965
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951