ADRENOCORTICOTROPIC SUBSTANCES FROM HUMAN PLACENTA1

Abstract
Four fractions obtained from human placental tissue and from blood contained in the intervillous space were assayed for adrenocorticotropic activity by the ascorbic acid depletion method. Fractions D and B, which were prepared by the oxycellulose technique from dried chorionic villi and from intervillous blood respectively, showed a striking corticotropic activity. The activity of 0.15 mg of fraction D was found to be comparable to that of 1 milliunit of the standard USP corticotropin.