Cultured mammary carcinoma cells contain gonadotropin‐releasing hormone‐like immunoreactivity, gnrh binding sites and chorionic gonadotropin

Abstract

Immunoperoxidase staining and radioimmunoassay were used to identify gonadotropin‐releasing hormone (GnRH) and chorionic gonadotropin (hCG) in 4 mammary carcinoma cell lines. GnRH‐like immunoreactivity was found in all cell lines; 2 lines also contained hCG. Binding of a GnRH agonist was demonstrated by radioreceptor assay in cultured mammary carcinoma cells. In cell column perfusion of the same cells, exogenous GnRH did not stimulate release of hCG.