SELYE (1946) and Long (1946) have reviewed the responses of the organism to various types of stress. The role of the anterior pituitary and the adrenal hormones in the adaptation phenomena are generally accepted. Decreases in the cholesterol and ascorbic acid content of the adrenals after (1) injection of adrenotrophic hormone (Sayers, Sayers, Fry, White and Long, 1944), (2) hemorrhage (Sayers, Sayers, Liang and Long, 1944), (3) scalding (Harkins and Long, 1945) (Harkins, 1945) and (4) exposure to cold (Levin, 1945) (Long, 1946) are characteristic changes noted a few hours after injury. A decrease in adrenal cholesterol content and concentration occurs a few days after trauma (Popjack, 1944) and after injection of β-chloroethyl vesicants (Ludewig and Chanutin, 1946). The latter investigators pointed out that the decrease in ester cholesterol is responsible for the drop in total cholesterol concentration. The present experiments were carried out to determine the changes in adrenal cholesterol and ascorbic acid after a variety of stresses over a period of several days.