The detergent solubility properties of a malarial (Plasmodium knowlesi) variant antigen expressed on the surface of infected erythrocytes
- 1 January 1984
- journal article
- research article
- Published by Wiley in Journal of Cellular Biochemistry
- Vol. 24 (3), 297-306
- https://doi.org/10.1002/jcb.240240310
Abstract
Four detergents have been compared for identification of the Plasmodium knowlesi variant antigen on infected erythrocytes by immunoprecipitation analysis. Erythrocytes infected with late trophozoite and schizont forms of cloned asexual parasites were labeled by lactoperoxidase‐catalyzed radioiodination and extracted either with the anionic detergents sodium dodecyl sulfate (SDS) or cholate, the neutral detergent Triton X‐100, or the zwitterion 3‐[(3‐cholamidopropyl)di‐methylammonio]‐1‐propane sulfonate (CHAPS). After addition of Triton X‐100 to SDS and cholate extracts, parallel immunoprecipitations of the four extracts were performed using rhesus monkey antisera of defined agglutinability. Identical results were obtained with clone Pkl(A+ ), which has 125I‐variant antigens of Mr 210,000 and 190,000, and with clone Pkl(B+)l+, which hasvariant antigens of Mr 200,000–205,000. SDS yielded maximal levels of immunoprecipitated 125I‐variant antigens. Variant‐specific immunoprecipitation was detected in some experiments with Triton X‐100 and cholic acid but with significantly lower recovery than with SDS. CHAPS extraction did not yield the variant antigens on immunoprecipitation. The variant antigens could also be identified in Triton X‐100‐insoluble material by subsequent extraction with SDS, indicating that failure to recover these proteins in the Triton X‐100‐soluble fraction is due to failure of this detergent to extract the variant antigens rather than to degradation during extraction. We suggest that the 125I‐variant antigens either have a structure that renders them intrinsically insoluble in Triton X‐100, cholate, or CHAPS, or that they are associated in some way with host cell membrane components that also resist solubilization by these detergents.Keywords
This publication has 14 references indexed in Scilit:
- Plasmodium falciparum strain-specific antibody blocks binding of infected erythrocytes to amelanotic melanoma cellsNature, 1983
- Surface alterations of erythrocytes in Plasmodium falciparum malaria. Antigenic variation, antigenic diversity, and the role of the spleen.The Journal of Experimental Medicine, 1983
- Radioiodination of new protein antigens on the surface of Plasmodium knowlesi schizont-infected erythrocytesMolecular and Biochemical Parasitology, 1982
- Antigenicity of the infected-erythrocyte and merozoite surfaces in Falciparum malaria.The Journal of Experimental Medicine, 1979
- Integral membrane protein interaction with triton cytoskeletons of erythrocytesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1979
- Two Plasmodium knowlesi-specific antigens on the surface of schizont-infected Rhesus monkey erythrocytes induce antibody production in immune hosts.The Journal of Experimental Medicine, 1979
- Maturation of the head of bacteriophage T4Journal of Molecular Biology, 1973
- Protective Immunity to Malaria provides a Model for the Survival of Cells in an Immunologically Hostile EnvironmentNature, 1971
- Immunity to Malaria: Antigenic Variation in Chronic Infections of Plasmodium knowlesiNature, 1965
- THE AGGLUTINATION OF PLASMODIUM KNOWLESI BY IMMUNE SERUMThe Journal of Experimental Medicine, 1938