Variations in the Specific Activity of Ribulose-1,5-bisphosphate Carboxylase between Species Utilizing Differing Photosynthetic Pathways

Abstract

The in vitro specific activity of ribulose-1,5-bisphosphate carboxylase (RuBPCase) (micromoles CO₂ fixed per minute per milligram enzyme) from a number of C₃ and C₄ species and one green alga were measured. RuBPCases from species which utilize the C₄ pathway have a specific activity ∼2-fold higher than those from C₃ species. RuBPCase from Chlamydomonas reinhardtii has a specific activity similar to the C₄ enzyme. Higher specific activity forms of RuBPCase are associated with a decreased enzyme affinity for CO₂ (increased K_m[CO₂]). A small but significant difference in the specific activity of RuBPCase from two C₄ decarboxylation types was also observed. The relationship between enzymic properties and the presence or absence of a CO₂ concentrating mechanism is discussed.