Online RP-HPLC-DPPH Screening Method for Detection of Radical-Scavenging Phytochemicals from Flowers of Acacia confusa
- 29 December 2007
- journal article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 56 (2), 328-332
- https://doi.org/10.1021/jf072314c
Abstract
Acacia confusa is traditionally used as a medicinal plant in Taiwan. In this study, phytochemicals and antioxidant activities of extracts from flowers of A. confusa were investigated for the first time. In addition, a rapid screening method, online RP-HPLC-DPPH system, for individual antioxidants in complex matrices was developed. Accordingly, six antioxidants including gallic acid (1), myricetin 3-rhamnoside (2), quercetin 3-rhamnoside (3), kaempferol 3-rhamnoside (4), europetin 3-rhamnoside (5), and rhamnetin 3-rhamnoside (6) were detected using the developed screening method. Of these, compounds 2, 3, and 5 were found to be major bioactive phytochemicals, and their contents were determined as 11.3, 6.7, and 8.7 mg/g of crude extract, respectively. By comparison with quercetin, a well-known antioxidant, these compounds had the order of compound 2 > compound 5 > quercetin > compound 3 for DPPH radical-scavenging activity. Their IC50 values were 3.0, 3.2, 4.5, and 7.4 µM, respectively. Moreover, the same order was observed for superoxide radical-scavenging activity, and their IC50 values were 2.6, 2.7, 4.3, and 5.3 µM, respectively. However, for lipid peroxidation, quercetin, an aglycon, showed the best inhibitory activity. The IC50 values of quercetin, compound 2, compound 5, and compound 3 were 46.7, 88.5, 90.7, and 124.6 µM, respectively. These results indicated that a rhamnoside at the C3 position of flavonoids had a negative effect on radical-scavenging activity and antilipid peroxidation. In contrast, the number of hydroxyl groups on the B-ring exhibited a positive relationship with their inhibitory activities.Keywords
This publication has 26 references indexed in Scilit:
- Antioxidant activities of natural phenolic compounds from Acacia confusa barkBioresource Technology, 2007
- A Review of the bioactivity and potential health benefits of chamomile tea (Matricaria recutita L.)Phytotherapy Research, 2006
- Phenolic Antioxidants from the Heartwood of Acacia confusaJournal of Agricultural and Food Chemistry, 2005
- Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancerLife Sciences, 2004
- Phenolics and Betacyanins in Red Beetroot (Beta vulgaris) Root: Distribution and Effect of Cold Storage on the Content of Total Phenolics and Three Individual CompoundsJournal of Agricultural and Food Chemistry, 2000
- An On-Line HPLC Method for Detection of Radical Scavenging Compounds in Complex MixturesAnalytical Chemistry, 2000
- Antioxidant Activity of Extracts from Du-zhong (Eucommia ulmoides) toward Various Lipid Peroxidation Models in VitroJournal of Agricultural and Food Chemistry, 1998
- Tannins and Human Health: A ReviewCritical Reviews in Food Science and Nutrition, 1998
- Antioxidant properties of phenolic compoundsTrends in Plant Science, 1997
- Oxidant stress and carcinogenesisEuropean Journal of Clinical Investigation, 1991