Cloning and Expression Patterns of two Members of A Novel Protein‐kinase‐C‐related Kinase Family
- 1 January 1995
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 227 (1-2), 344-351
- https://doi.org/10.1111/j.1432-1033.1995.tb20395.x
Abstract
The cDNA clones for two members of a novel protein kinase family were isolated and sequenced. These protein-kinase-C-related kinases, PRK1 and PRK2, display extensive identity to each other, revealing non-kinase domain similar regions. HR1 and HR2. HR1 contains a motif repeated three times (HR1a-c), while HR2 shows similarity to the amino-terminal sequence of protein-kinase-C epsilon and eta isotypes. Both PRK1 and PRK2, expressed in COS 1 cells, are autophosphorylated in immunoprecipitates, indicating intrinsic kinase activity. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues.Keywords
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