The quantitative estimation of 3:4-benzpyrene in whole animals, their tissues and excreta
- 1 February 1942
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 36 (1-2), 86-91
- https://doi.org/10.1042/bj0360086
Abstract
A method is described for the quantitative estimation of 3:4-benzpyrene in whole mice, separate tissues, tissue fluids and excreta. It is based on the "wet" method of extraction and spectrographic analysis of the fluorescence of the extract, originally described by Berenblum & Kendal (1936) for the estimation of 1:2:5:6:-dibenzanthracene. The most important changes in the present method are concerned with the purification of the extract for the removal of interfering substances. These result in a considerable simplification of the procedure and a greater sensitivity. Limit of sensitivity: about 0.05 [gamma] per mouse; accuracy: about [plus or minus]20% over a range of 1-10,000 [gamma] per mouse.This publication has 3 references indexed in Scilit:
- The rate of disappearance of 3:4-benzpyrene from the mouse after subcutaneous and intraperitoneal injectionBiochemical Journal, 1942
- The role of the 1:2:5:6-dibenzanthracene in the production of fowl tumoursBiochemical Journal, 1934
- The spectra of cancer-producing tars and oils and of related substancesBiochemical Journal, 1930