Synthesis and thermal melting behavior of oligomer.polymer complexes containing defined lengths of mismatched dA·dG and dG·dG nucleotides
- 1 May 1977
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 16 (11), 2367-2374
- https://doi.org/10.1021/bi00630a009
Abstract
Model DNA polymers containing heteroduplex regions of defined sequence and size were synthesized using polynucleotide phosphorylase and calf thymus terminal transferase. Heteroduplexes were of the form (dG)n .cntdot. d(C12AmC.hivin.x), where m = 1-6, and (dG)n .cntdot. d(C10GmC.hivin.x), where m = 1 and 3-5 [.hivin.x = average length of the block of dC residues]. Thermal melting studies of the model DNA indicated that the heteroduplex regions did not disrupt the cooperative interaction between the flanking regions of dG .cntdot. dC base pairs. Thus, it is possible that the heteroduplex nucleotides are accommodated in a stacked helical structure.This publication has 7 references indexed in Scilit:
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