The Need For Modification of the Polyvinyl Sponge Model of Connective Tissue Growth: Histologic and Biochemical Studies in the Rabbit
- 1 January 1975
- journal article
- research article
- Published by Taylor & Francis in Connective Tissue Research
- Vol. 3 (4), 213-225
- https://doi.org/10.3109/03008207509152181
Abstract
Quantitative histopathologic and biochemical comparisons were made between polyvinyl sponge capsular and sponge tissue in the rabbit on different days after subcutaneous implantation. Up to 9 days the predominant cell type in the capsular tissue is the fibroblast and in the sponge it is the neutrophil. During this time period the sponge tissue shows lower rates of (14C) proline and (14C) cytidine incorporation and lower rates of total (14C) collagen synthesis than the surrounding capsule. Different gel electrophoretic patterns of isolated radioactive proteins are found in sponge and capsule at 6 days. These biochemical differences appear to be related to the small number of fibroblasts, relative to granulocytes present in sponges during the first 9 days after implantation. It is suggested that future biochemical investigations of the early phase of connective tissue reactions (first 9 days) in this model utilize sponge capsular tissue within 1 cm of the sponge edge instead of the sponge and its contents.Keywords
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