Mucopolysaccharide, Protein and Desoxyribosenucleic Acid Concentration of Granulation Tissue Induced by Polyvinyl Sponges.

Abstract

Polyvinyl sponges inserted under the dorsal skin of guinea pigs fill with vascular granulation tissue containing fibroblasts and few giant cells, but no inflammatory infiltrate. The dry weight of the granulation tissue entering the sponges reaches a maximum of 211 mg (mean) at 10 days, then falls progressively. The protein content reaches a maximum of 14.5 g/100 g, the desoxyribosenucleic acid content 986 mg/100 g, and the mucopolysaccharide content 192 mg/100 g (as uronic acid). The carbazole/orcinol ratio of the uronic acid moiety fell progressively after 10 days, suggesting a relative increase in chondroitin sulfate B. Both glucosamine and galactosamine were found in the mucopolysaccharide fraction, as was chromatographic suggestion of the presence of chondroitin sulfate A and hyaluronate.