Pancreatic and Gastric Secretion in Rats Studied by Means of Duodenal and Gastric Perfusion

Abstract

The duodenum and stomach of female rats, anaesthetized by urethane, were perfused separately and continuously with saline solution. Stimulation was performed by means of intravenous infusion of secretin, cholecystokinin-pancreozymin (CCK-PZ), and pentagastrin in a ‘step-test’, increasing the infused dosages in 20-minute periods. During basal secretion only acid and bicarbonate were detectable. High amounts of CCK-PZ induced the same maximal acid output as pentagastrin. All three hormones evoked the bicarbonate output in different intensities. The output of pancreatic enzymes was influenced only by CCK-PZ and pentagastrin. During the course of stimulation amylase showed a constant secretion rate. On the other hand, trypsin and chymotrypsin output increased to a peak and then decreased. In contrast to man and the dog, rather high doses of the different hormones are necessary to reach maximal secretion of acid, bicarbonate, and enzymes.