On the Regenerative Capacity of Human Skeletal Muscle
- 1 March 2005
- journal article
- Published by Wiley in Artificial Organs
- Vol. 29 (3), 192-198
- https://doi.org/10.1111/j.1525-1594.2005.29033.x
Abstract
The proliferative capacity of organotypic muscle stem cells, the satellite cells, from nine healthy human donors aged between 2 and 78 years was investigated. There was a loss in proliferative capacity with age, but the oldest donors (76, 78 years) would still be able to replace their musculature several times. Depending on frequency of desmin-positive (i.e., myogenic) cells during prolonged expansion, myoblast cultures could be designated as stable or unstable. There was a weak correlation between mean telomere lengths (estimated by flow-FISH) and remaining mean population doublings until senescence. A bimodal distribution of mean telomere lengths was apparent in both stable and unstable myoblast cultures and could be assigned to populations of differently sized cells. Furthermore, due to the presence of nonmyogenic cells with longer telomeres, unstable cultures tended to show an increasing rather than decreasing mean telomeric length on expansion. Bimodal distributions in myoblast cultures could be due to hitherto undefined myoblast populations.Keywords
This publication has 24 references indexed in Scilit:
- Insulin-like Growth Factor-I Extends in VitroReplicative Life Span of Skeletal Muscle Satellite Cells by Enhancing G1/S Cell Cycle Progression via the Activation of Phosphatidylinositol 3′-Kinase/Akt Signaling PathwayJournal of Biological Chemistry, 2000
- Role of Telomerase in Normal and Cancer CellsJournal of Clinical Oncology, 2000
- Accumulation of Short Telomeres in Human Fibroblasts Prior to Replicative SenescenceExperimental Cell Research, 2000
- Replicative Potential and Telomere Length in Human Skeletal Muscle: Implications for Satellite Cell-Mediated Gene TherapyHuman Gene Therapy, 1997
- Mechanical function of dystrophin in muscle cells.The Journal of cell biology, 1995
- Telomere length predicts replicative capacity of human fibroblasts.Proceedings of the National Academy of Sciences, 1992
- Normal myogenic cells from newborn mice restore normal histology to degenerating muscles of the mdx mouse.The Journal of cell biology, 1990
- Accelerated age-related decline in replicative life-span of Duchenne muscular dystrophy myoblasts: Implications for cell and gene therapySomatic Cell and Molecular Genetics, 1990
- Conversion of mdx myofibres from dystrophin-negative to -positive by injection of normal myoblastsNature, 1989
- Muscle damage and repair in voluntarily running mice: strain and muscle differencesCell and tissue research, 1987