Wheat plants regenerated from embryo cell cultures.

Abstract

Wheat cultures capable of plant regeneration were obtained from young embryos of T. aestivum cv. Chinese Spring and cv. Salmon, and were maintained by subculturing them every 30-40 days on RM-64 basal medium supplemented with 2.0 mg/l of 2,4-D. Embryos (14 days old) were at the optimum age for the induction of cultures capable of plant regeneration. Histological observations showed many shoot apices on the surface of these cultures. The cultures remained capable of differentiation for .apprx. 8 mo. Cultures from wheat embryos may be useful in mutant selection studies.