DNA supercoiling by Xenopus laevis oocyte extracts: requirement for a nuclear factor.
- 1 October 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (10), 4873-4876
- https://doi.org/10.1073/pnas.75.10.4873
Abstract
A purified system is described for the introduction of negative supercoils into sv 40 DNA. The system consists of histones H2A, H2B, H3 and H4, DNA-relaxing enzyme, and a purified factor from X. laevis stage 6 oocyte nuclei. The nuclei are prepared en masse by the technique of F. Scalenghe, et al (1978). The supercoiled sv-40 DNA prepared by this method is indistinguishable from sv-40 supercoiled DNA prepared from infected African green monkey [cv-1] cells.This publication has 17 references indexed in Scilit:
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