Analysis of N‐acetyl‐4‐O‐acetylneuraminic‐acid‐containing. N‐linked carbohydrate chains released by peptide‐N4‐(N‐acetyl‐β‐glucosaminyl)asparagine amidase F

Abstract
The carbohydrate chains of equine fibrinogen were enzymatically released by peptide-N4-(N-acetyl-.beta.-glucosaminyl)asparagine amidase F. The oligosaccharides obtained were fractionated by a combination of FPLC and HPLC and analyzed by 500-MHz 1H-NMR spectroscopy. Four monosialo and four disialo diantennary N-acetyllactosamine type of carbohydrate chains occur.