Primary structure of two major glycans of bovine fibrinogen

Abstract
After pronase digestion of bovine fibrinogen, the asparagine-linked glycans were released from the resulting glycopeptides by hydrazinolysis, and subsequently re-N-acetylated. Two sialylated glycans were isolated by ion exchange chromatography. Their primary structure has been determined by methylation analysis and 360-MHz 1H-NMR spectroscopy. The structures proposed to be present in the native glycoprotein are as follows: .**GRAPHIC**. .**GRAPHIC**.