INVITRO AND INVIVO STIMULATION OF NEUTROPHIL MIGRATION AND LYMPHOCYTE-TRANSFORMATION BY THIAMINE RELATED TO INHIBITION OF THE PEROXIDASE-H2O2-HALIDE SYSTEM
- 1 January 1981
- journal article
- research article
- Vol. 44 (2), 295-303
Abstract
The effects of thiamine [TM] on [human] neutrophil [NP] functions and mitogen-induced lymphocyte transformation were investigated in vitro and in vivo in adult volunteers following the injection of 50 mg thiamine i.m. TM caused stimulation of NP motility in vitro and in vivo and increased lymphocyte transformation in vivo. Enhancement of these functions was related to inhibition of NP post-phagocytic iodination of Candida albicans by the myeloperoxidase/H2O2/halide system. The horseradish peroxidase[HPO]/H2O2/125I-mediated iodination of bovine serum albumin was also inhibited by TM concentrations which caused increased neutrophil motility. Preincubation of NP and lymphocytes with the HPO/H2O2/halide system caused considerable inhibition of the migratory and proliferative responses, respectively. Inclusion of TM at concentrations which inhibited the peroxidase/H2O2/halide system protected the NP migratory and lymphocyte proliferative responses from inactivation by this system. Apparently TM may cause increased NP migration and lymphocyte transformation by protecting these cells from toxic oxidative products generated by the peroxidase/H2O2/halide system.This publication has 13 references indexed in Scilit:
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