A METHOD FOR THE SIMULTANEOUS DETERMINATION OF 17-OXOSTEROIDS AND 17-HYDROXYCORTICOSTEROIDS IN HUMAN PLASMA
- 1 February 1964
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 28 (3), 289-296
- https://doi.org/10.1677/joe.0.0280289
Abstract
SUMMARY: A simple method for the simultaneous estimation of 17-hydroxycorticosteroids and 17-oxosteroids in human plasma is described. It involves extraction of free corticosteroids, hydrolysis of glucuronosides with β-glucuronidase and solvolysis of sulphates, chromatography on silica gel and colorimetry using microcells. Recoveries of added steroids, using appropriate conjugated compounds were over 80%.Keywords
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