A METHOD FOR THE SIMULTANEOUS ASSAY OF 17-HYDROXYCORTICOSTEROIDS AND 17-KETOSTEROIDS BOTH IN THEIR FREE AND CONJUGATED FORMS ON THE SAME PLASMA SAMPLE
- 1 November 1958
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 29 (3), 321-333
- https://doi.org/10.1530/acta.0.0290321
Abstract
By this method 17-hydroxycorticosteroids (17-OH-CS) can be selectively extracted from a chloroform solution by a phenylhydrazine sulfuric acid reagent while the 17-ketosteroids (17-KS) remain in the chloroform from which they can be quantitatively recovered. Reproducibility and recovery experiments show that the values obtained with this method are significant to a limit lower than [plus or minus] 10%. Preliminary attempts at a chroma-tographic partition of plasma steriods are also reported.Keywords
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