Agrobacterium tumefaciens-mediated transformation of Pisum sativum L. using binary and cointegrate vectors
- 1 September 1990
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 9 (5), 276-279
- https://doi.org/10.1007/bf00232301
Abstract
Epicotyl segments and nodus expiants from etiolated seedlings of Pisum sativum were transformed using Agrobacterium tumefaciens strains GV 2260 (p35S GUS INT) and GV 3850 HPT carrying either a neomycin- or hygromycinphosphotransferase-gene as selectable markers. The transgenic character of hygromycin- or kananamycin-resistant tissue was confirmed by detection of nopaline or neomycinphosphotransferase-II- and ß-glucuronidase activity in crude extracts of resistant tissues. Up to 5 % of developing shoots from shoot proliferating nodi were regenerated via organogenesis to kanamycin-resistant plantlets. Transformation frequency in vitro was found to be influenced by expiant source, A. tumefaciens strain, pea genotype and duration of cocultivation. Acetosyringone did not increase the transformation rate.Keywords
This publication has 16 references indexed in Scilit:
- Construction of an intron-containing marker gene: Splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformationMolecular Genetics and Genomics, 1990
- Plant regeneration from pea protoplasts via somatic embyogenesisPlant Cell Reports, 1989
- Specificity of strain and genotype in the susceptibility of pea to Agrobacterium tumefaciensPlant Cell Reports, 1989
- Origin of somatic embryos from repetitively embryogenic cultures of walnut (Juglans regia L.): Implications forAgrobacterium-mediated transformationPlant Cell Reports, 1989
- Parameters affecting the frequency of kanamycin resistant alfalfa obtained by Agrobacterium tumefaciens mediated transformationPlant Cell Reports, 1988
- Plant regeneration via somatic embryogenesis in pea (Pisum sativum L.)Plant Cell Reports, 1987
- The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNAJournal of Bacteriology, 1986
- Efficient octopine Ti plasmid-derived vectors forAgrobacterium-mediated gene transfer to plantsNucleic Acids Research, 1985
- A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activitiesBiochimica et Biophysica Acta (BBA) - Enzymology, 1978
- A Plant-Tumor of Bacterial OriginScience, 1907