Die Erzeugung von Mutanten des Tabakmosaikvirus durch den Einbau von Fluoruracil in die Virusnucleinsäure

Abstract

Tobacco mosaic virus samples in which 17, 36 and 56% of the uracil (U) was replaced by 5-fluorouracil (FU) were prepared. The samples were tested for the presence of mutants on various varieties of Nicotiana tabacum. The virus containing 17% FU showed no detectable increase, whereas the samples with 36% and 56% substitution of U by FU showed a 5 -10 fold increase in the number of mutants in comparison with appropriate controls. Evidence is presented that the observed increase is not due to a selection.