Evidence that biosynthesis of platelet‐activating factor (paf‐acether) by human neutrophils occurs in an intracellular membrane
- 28 October 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 191 (2), 195-199
- https://doi.org/10.1016/0014-5793(85)80007-7
Abstract
Human polymorphonuclear leukocytes (PMN) were incubated in the absence or presence of the calcium ionophore A23187 (6μM) for 10 min at 37°C. They were then lysed by nitrogen cavitation and fractionated using Percoll gradients. Three major fractions of increasing density corresponding to plasma membrane, intracellular membranes and secretory granules were detected using [3H]concanavalin A, NADH-dehydrogenase and β-D-glucuronidase as respective markers. In both cases, the acetyltransferase activity responsible for biosynthesis of paf-acether (platelet-activating factor of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) was detected in the intermediary fraction, the enzyme activity being increased 3-4-fold in stimulated cells. From the comparison with the distribution of various markers, it is concluded that in human PMN the final step of paf-acether assembly occurs in an intracellular membrane, possibly the endoplasmic reticulum.Keywords
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