SEPARATION OF OPIOID PEPTIDES UTILIZING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
- 12 January 2009
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 13 (5), 493-497
- https://doi.org/10.1111/j.1399-3011.1979.tb01911.x
Abstract
Chromatographic procedures were developed for resolving all of the known enkephalins and endorphins on a single column. The effect of eluant pH on the retention times and separation of the enkephalins and .beta.-endorphin was determined. By combining these separations with a sensitive radioreceptor assay it is possible to assay all of the opioid peptides in the pituitary gland or in various regions of the brain from individual small laboratory animals.Keywords
This publication has 11 references indexed in Scilit:
- On βH-Leu5-Endorphin and SchizophreniaArchives of General Psychiatry, 1979
- Met-enkephalin: Rapid separation from brain extracts using high-pressure liquid chromatography, and quantitation by binding assayNeurochemical Research, 1978
- Rapid purification of β-endorphin by high-performance liquid chromatographyJournal of Chromatography A, 1978
- Brain enkephalin distribution is unaltered by hypophysectomyLife Sciences, 1978
- Incorporation of labelled amino acids into the enkephalinsFEBS Letters, 1977
- THE DISTRIBUTION OF METHIONINE‐ENKEPHALIN AND LEUCINE‐ENKEPHALIN IN THE BRAIN AND PERIPHERAL TISSUESBritish Journal of Pharmacology, 1977
- Endorphins in several regions of rat brain: Large differences between radioimmunoassay and radioreceptor assayEuropean Journal of Pharmacology, 1977
- Antibodies as a means of isolating and characterizing biologically active substances: presence of a non-peptide, morphine-like compound in the central nervous system.Proceedings of the National Academy of Sciences, 1976
- Identification of two related pentapeptides from the brain with potent opiate agonist activityNature, 1975
- Automatic monitoring of primary amines in preparative column effluents with fluorescamineAnalytical Biochemistry, 1975