GABAAand GABABreceptors are functionally active in the regulation of catecholamine secretion by bovine chromaffin cells
- 1 July 1989
- journal article
- research article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 23 (3), 290-296
- https://doi.org/10.1002/jnr.490230307
Abstract
GABA stimulates the basal catecholamine release from adrenal bovine chromaffin cells in a calciumdependent manner. This release represents about 70% of that obtained by similar doses of nicotine under similar experimental conditions. This effect is mediated by GABAA receptor sites present in chromaffin cells, since it was mimicked by muscimol and reversed by bicuculline. In addition, GABA, through its GABAA receptors, increases the catecholamine release evoked by submaximal doses of aicotine, but it has no effect on nicotine‐evoked secretion of catecholamines when nicotine was given at maximal doses. These results seem to indicate that both nicotine and GABA release catecholamines from the same intracellular pool. In contrast, baclofen, a GABAB receptor agonist, depressed both basal and nicotineevoked catecholamine release; this result indicates that in addition to GABAA control of catecholamine secretion by chromaffin cells, there is a GABAB control of this function. These results support the existence of a dual regulation of catecholamine secretion by both the GABAA and GABAB receptors in a similar way as that proposed for muscarinic and nicotinic cholinergic receptors.Keywords
This publication has 22 references indexed in Scilit:
- Stimulatory Action of ?-Aminobutyric Acid on Catecholamine Secretion from Bovine Adrenal Chromaffin Cells Measured by a Real-Time Monitoring SystemJournal of Neurochemistry, 1988
- GABAA and GABAB sites in bovine adrenal medulla membranesJournal of Neuroscience Research, 1988
- Dihydropyridine Modulation of the Chromaffin Cell Secretory ResponseJournal of Neurochemistry, 1987
- Real‐Time Monitoring of the Secretory Function of Cultured Adrenal Chromaffin CellsJournal of Neurochemistry, 1986
- GABA and bicuculline actions on mouse spinal cord and cortical neurons in cell cultureBrain Research, 1982
- Molecular cloning establishes proenkephalin as precursor of enkephalin-containing peptidesNature, 1982
- Immunocytochemical Study of Microtubules in Chromaffin Cells in Culture and Evidence That Tubulin Is Not an Integral Protein of the Chromaffin Granule MembraneJournal of Neurochemistry, 1981
- Modulation of nicotinic receptors by opiate receptor agonists in cultured adrenal chromaffin cellsNature, 1980
- Cholinergic muscarinic receptors of bovine adrenal medullaNeuropharmacology, 1979
- Rapid rise in cyclic GMP accompanies catecholamine secretion in suspensions of isolated adrenal chromaffin cellsLife Sciences, 1979