Epigenetically Reprogramming Metastatic Tumor Cells With An Embryonic Microenvironment
- 3 December 2009
- journal article
- Published by Taylor & Francis in Epigenomics
- Vol. 1 (2), 387-398
- https://doi.org/10.2217/epi.09.25
Abstract
We have previously shown that the microenvironment of human embryonic stem cells (hESCs) is able to change and reprogram aggressive cancer cells to a less aggressive state. Some mechanisms implicated in the phenotypic changes observed after this exposure are mainly associated with the Nodal signaling pathway, which plays a key role in tumor cell plasticity. However, several other molecular mechanisms might be related directly and/or indirectly to these changes, including microRNA (miRNA) regulation and DNA methylation. Aim: To further explore the epigenetic mechanisms potentially underlying the phenotypic changes that occur after exposing metastatic melanoma cells to a hESC microenvironment. Materials & Methods: A total of 365 miRNAs were screened using the TaqMan® Low Density Arrays. We also evaluated whether DNA methylation could be one of the factors regulating the expression of the inhibitor of Nodal, Lefty, in hESCs (where it is highly expressed) vs melanoma cells (where it is not expressed). Results...Keywords
Funding Information
- National Institutes of Health (CA59702, CA121205)
- Eisenberg Scholar Research Fund
- Maeve McNicholas Memorial Foundation
This publication has 38 references indexed in Scilit:
- Modulation of potassium channel function confers a hyperproliferative invasive phenotype on embryonic stem cellsProceedings of the National Academy of Sciences, 2008
- Human embryonic stem cell microenvironment suppresses the tumorigenic phenotype of aggressive cancer cellsProceedings of the National Academy of Sciences, 2008
- Epidemiology of Melanoma and Nonmelanoma Skin Cancer—The Role of SunlightAdvances in Experimental Medicine and Biology, 2007
- Treatments for metastatic melanoma: Synthesis of evidence from randomized trialsCancer Treatment Reviews, 2007
- Reprogramming metastatic tumour cells with embryonic microenvironmentsNature Reviews Cancer, 2007
- Targeting Nodal in malignant melanoma cellsEmerging Therapeutic Targets, 2007
- The Epigenomics of CancerCell, 2007
- Non-coding RNAs: Lost in translation?Gene, 2007
- Nodal Signaling in Vertebrate DevelopmentAnnual Review of Cell and Developmental Biology, 2003
- Structural conservation of Notch receptors and ligandsSeminars in Cell & Developmental Biology, 1998