Activation of nif gene expression in Azotobacter by the nifA gene product of Klebsiella pneumoniae
- 1 February 1983
- journal article
- Published by Springer Nature in Nature
- Vol. 301 (5901), 626-628
- https://doi.org/10.1038/301626a0
Abstract
Structural similarity of nitrogenase, the enzyme responsible for biological nitrogen fixation, from various diazotrophic bacteria has been shown by intergeneric biochemical complementation of component proteins in vitro, DNA and protein sequence analysis, and DNA hybridization between nif (nitrogen fixation) structural genes from Klebsiella pneumoniae and genomic sequences from other nitrogen-fixing bacteria. Despite nitrogenase homologies, little is known about similarities among nif regulatory mechanisms although repression of nitrogenase synthesis by NH4+ and O2 occurs in most diazotrophs. In K. pneumoniae, the ntr (gln) genes concerned with regulation of nitrogen metabolism control expression of the nifLA operon whose products act as repressor (nifL) and activator (nifA) of the seven other nif transcriptional units. Here we report that the nifA gene product of K. pneumoniae can activate expression of nif genes in both Azotobacter vinelandii and Azotobacter chroococcum, organisms whose aerobic physiology contrasts with that of the facultative K. pneumoniae.Keywords
This publication has 27 references indexed in Scilit:
- Complementation analysis of glnA-linked mutations which affect nitrogen fixation in Klebsiella pneumoniaeMolecular Genetics and Genomics, 1981
- The cloning and transposon Tn5 mutagenesis of the glnA region of Klebsiella pneumoniae: Identification of glnR, a gene involved in the regulation of the nif and hut operonsMolecular Genetics and Genomics, 1981
- Correspondence of the Larger Subunit of the MoFe-Protein in Clostridial Nitrogenase to the nif D Gene Products of Other N2-Fixing Organisms1The Journal of Biochemistry, 1981
- Nitrogen fixation gene (nifL) involved in oxygen regulation of nitrogenase synthesis in K. peumoniaeNature, 1981
- Nucleotide sequence of the R.meliloti nitrogenase reductase (nifH) geneNucleic Acids Research, 1981
- A nif mutant of Klebsiella pneumoniae fixing nitrogen in the presence of ammoniaFEMS Microbiology Letters, 1981
- Nucleotide sequence of a cyanobacterial nifH gene coding for nitrogenase reductaseProceedings of the National Academy of Sciences, 1980
- Comparison of the iron proteins from the nitrogen fixation complexes of Azotobacter vinelandii, Clostridium pasteurianum , and Klebsiella pneumoniaeProceedings of the National Academy of Sciences, 1980
- Interspecies homology of nitrogenase genes.Proceedings of the National Academy of Sciences, 1980
- Identification of blue-green algal nitrogen fixation genes by using heterologous DNA hybridization probesProceedings of the National Academy of Sciences, 1980