Intracellular Localization of Polyoma Virus Antigen Demonstrated With Fluoresce in Labeled Antiserums

Abstract

The γ-globulin fraction of antiserums prepared in rabbits against polyoma virus, grown in milk-adapted P388 D_i cells, was labeled with fluorescein isothiocyanate. When this labeled serum was applied to polyoma virus-infected P388 D₁ tissue-culture cells and examined under ultraviolet light, particulate fluorescent areas were demonstrated in the nuclei. These fluorescent particles were not seen in the uninfected tissue-culture preparations. These findings are interpreted as demonstrating intranuclear virus or intranuclear viral-associated antigen in cells infected with polyoma virus.