Characterization of "High-Affinity"[3H]Ouabain Binding in the Rat Central Nervous System
- 1 June 1985
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 44 (6), 1709-1715
- https://doi.org/10.1111/j.1471-4159.1985.tb07158.x
Abstract
The characteristics of [3H] ouabain binding were examined in various areas of rat brain. In the striatum, Scatchard analysis revealed a single class of high-affinity binding sites with an apparent binding affinity (Kd) of 10.4 .+-. 0.9 n M and an estimated binding capacity (Bmax) of 7.6 .+-. 1.9 pmol/mg protein. Similar monophasic Scatchard plots were found in the brainstem, cerebellum, hypothalamus and frontal cerebral cortex. [3H] Ouabain binding to rat brain was Na- and ATP dependent and strongly inhibited by K. Proscillaridin A was the most potent cardiac glycoside tested in inhibiting specific. [3H] ouabain binding to brain membranes and the rank order of inhibitory potencies for a series of cardiac glycosides was similar to that for inhibition of heart Na, K-ATPase. To assess whether the high-affinity binding sites for [3H] ouabain were localized to neuronal or nonneuronal membranes, the effect of discrete kainic acid lesions on striatal [3H] ouabain binding was examined. Kainic acid lesions of the striatum reduced [3H] ouabain binding to striatal homogenates by 79.6 .+-. 1.6%. Evidently, the high-affinity binding of [3H] ouabain binding sites are localized to neuronal elements. Thus, the high-affinity binding of [3H] ouabain to brain membranes may selectively label a neuronal form or conformation of Na, K-ATPase.Keywords
This publication has 13 references indexed in Scilit:
- Characterization of [3H]Ouabain Binding Sites in Human Brain, Platelet, and ErythrocyteJournal of Neurochemistry, 1985
- High-affinity ouabain binding site and low-dose positive inotropic effect in rat myocardiumNature, 1982
- Cardiac glycoside receptor, (Na+ + K+)ATPase activity and force of contraction in rat heartBiochemical Pharmacology, 1980
- LIGAND: A versatile computerized approach for characterization of ligand-binding systemsAnalytical Biochemistry, 1980
- The characteristics of the binding of 12-alpha-[3H]-digoxin to the membranes of intact human erythrocytes: relevance to digoxin therapy.British Journal of Clinical Pharmacology, 1979
- A heterogeneous ouabain-ATPase interaction in mouse brainLife Sciences, 1978
- Kainic acid lesions of the striatum: behavioural sequalae similar to Huntington's ChoreaBrain Research, 1978
- Membrane Adenosinetriphosphatase: A Digitalis Receptor?Science, 1977
- Non-uniform populations of g-strophanthin binding sites of (Na+ + K+)-activated ATPase Apparent conversion to uniformity by K+Biochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- REGIONAL STUDIES OF CATECHOLAMINES IN THE RAT BRAIN‐IJournal of Neurochemistry, 1966