Characterization of [3H]Ouabain Binding Sites in Human Brain, Platelet, and Erythrocyte
- 1 June 1985
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 44 (6), 1704-1708
- https://doi.org/10.1111/j.1471-4159.1985.tb07157.x
Abstract
[3H]Ouabain binding was investigated in membranes prepared from human brain, erythrocyte, and platelet. Scatchard analysis of [3H]ouabain binding to human hypothalamic membranes revealed a single class of noninteracting binding sites with an apparent affinity constant (KD) of 21 nM. Though the number of [3H]ouabain binding sites was lower in human platelets than in erythrocytes, both tissues exhibited a single class of high‐affinity binding sites with an apparent KD similar to that found in human brain. Specific [3H]ouabain binding in basal ganglia tissue from patients with Huntington's disease was more than 50% lower than in tissue from age‐ and sex‐matched controls. These results, along with previous findings in rat brain, suggest that high‐affinity [3H]ouabain binding labels the neuronal form of Na,K‐ATPase in human brain, and may prove useful in quantitating this enzyme in postmortem brain samples.Keywords
This publication has 10 references indexed in Scilit:
- Characterization of "High-Affinity"[3H]Ouabain Binding in the Rat Central Nervous SystemJournal of Neurochemistry, 1985
- RBC Membrane Adenosine Triphosphatase Activities in Patients With Major Affective DisordersArchives of General Psychiatry, 1983
- High-affinity ouabain binding site and low-dose positive inotropic effect in rat myocardiumNature, 1982
- Erythrosin B is a specific inhibitor of high affinity 3H-ouabain binding and ion transport in rat brainNeuropharmacology, 1981
- Cardiac glycoside receptor, (Na+ + K+)ATPase activity and force of contraction in rat heartBiochemical Pharmacology, 1980
- Platelet and erythrocyte-membrane adenosine triphosphatase activity in depressive and manic-depressive illnessPsychiatry Research, 1980
- Demonstration of specific “high affinity” binding sites for [3H] imipramine on human plateletsLife Sciences, 1980
- Two molecular forms of (Na+ + K+)-stimulated ATPase in brain. Separation, and difference in affinity for strophanthidin.Journal of Biological Chemistry, 1979
- A heterogeneous ouabain-ATPase interaction in mouse brainLife Sciences, 1978
- Quantitative aspects of ouabain binding to human erythrocyte and cardiac membranes.The Journal of Physiology, 1975