A program for least squares analysis of reassociation and hybridization data
Open Access
- 1 June 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (6), 1727-1738
- https://doi.org/10.1093/nar/4.6.1727
Abstract
A computer program is described for the rapid calculation of least squares solutions for data fitted to different functions normally used in reassociation and hybridization kinetic measurements. The equations for the fraction not reacted as a function of Cot follow: First order, exp(−kCot); second order, (1+kCot)−1 ; variable order, (1+kCot)−n; approximate fraction of DNA sequence remaining single stranded, (1+kCot)−44; and a function describing the pairing of tracer when the rate constant for the tracer (k) is distinct from the driver rate constant kd): exp{kL1-(1+kdCot)1−nJ/Lkd(1-n)J}. Several components may be used for most of these functional forms. The standard deviations of the individual parameters at the solutions are calculated.Keywords
This publication has 10 references indexed in Scilit:
- Structural gene sets active in embryos and adult tissues of the sea urchinCell, 1976
- Studies on nucleic acid reassociation kinetics: empirical equations describing DNA reassociation.Proceedings of the National Academy of Sciences, 1976
- Studies on nucleic acid reassociation kinetics: reactivity of single-stranded tails in DNA-DNA renaturation.Proceedings of the National Academy of Sciences, 1975
- Complexity of cytoplasmic RNA in different mouse tissue measured by hybridization of polyadenylated RNA to complementary DNABiochemistry, 1975
- Structural genes adjacent to interspersed repetitive DNA sequencesCell, 1975
- Comparative aspects of DNA organization in metazoaChromosoma, 1975
- Three abundance classes in HeLa cell messenger RNANature, 1974
- A measurement of the sequence complexity of polysomal messenger RNA in sea urchin embryosCell, 1974
- Organization, Transcription, and Regulation in the Animal GenomeThe Quarterly Review of Biology, 1973
- Kinetics of renaturation of DNAJournal of Molecular Biology, 1968