An Acetic-Haematoxylin Squash Method for Small Chromosomes
Open Access
- 1 January 1968
- journal article
- research article
- Published by Firenze University Press in Caryologia
- Vol. 21 (2), 115-119
- https://doi.org/10.1080/00087114.1968.10796290
Abstract
The use of an acetic-haematoxylin solution in a squash method for small chromosomes is described. The concentration of the solution is 2% haematoxylin (Merck) in 45% acetic acid, the mordant is iron acetate. Three schedules are described: for Collembola or other small insects, for root tips and leaf buds, and for pollen mother cells. Hoyer's or modified Berlese's mounting media are employed to make slides permanent.Keywords
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- A Mordanting Fixation for Intense Staining of Small ChromosomesStain Technology, 1953
- Studies on Hematoxylin StainsStain Technology, 1943