A Mordanting Fixation for Intense Staining of Small Chromosomes
- 1 January 1953
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 28 (6), 305-308
- https://doi.org/10.3109/10520295309105561
Abstract
A combination iron-mordant fixative in which propionic acid is substituted for acetic acid has been found useful in preparing small plant chromosomes for carmine stained squashes. Propionic acid is better than acetic acid because it holds more iron in stable solution. The fixative is a 3:1 mixture of 95% alcohol and pure propionic acid which contains 400 mg. of Fe(OH)3 per 100 ml. of propionic acid. The latter is previously prepared by dissolving the dry freshly prepared Fe(OH)3 in it. To each 10 ml. vial of fixative is added a few drops of carmine stain. Standard aceto-carmine squashes of material fixed in this mixture show quick intense staining and are especially useful for differentiated chromosomes at mitotic prophase.Keywords
This publication has 8 references indexed in Scilit:
- A Controllable Carmine Technic for Plants with Small ChromosomesStain Technology, 1952
- Pachytene Morphology of the Tomato Chromosome ComplementAmerican Journal of Botany, 1950
- SOME MODIFICATIONS OF WARMKES PERMANENT SECTION-SMEAR METHOD FOR PLANT CHROMOSOMES1949
- Basic Fuchsin as a Nuclear StainStain Technology, 1949
- A Method for Making Aceto-Carmine Squashes Permanent Without Removal of the Cover SlipStain Technology, 1948
- Control of the Ferric Ion Concentration in Iron-Aceto-Carmine StainingStain Technology, 1944
- The Aceto-Carmine Method for Fruit MaterialStain Technology, 1940
- A Modified Root Tip Smear TechnicStain Technology, 1937