Mammalian glycosidases. 4. The intracellular localization of β-galactosidase, α-mannosidase, β-N-acetylglucosaminidase and α-l-fucosidase in mammalian tissues

Abstract

The intracellular localization of [beta]-galacto-sidase, [alpha]-mannosidase, [beta]-N-acetylglucosaminidase and [alpha]-L-fucosidase in some tissues of the mouse and the rat has been studied by centrifugal fractionation of homogenates in 0.25[image]-sucrose. All the glycosidases were particle-bound to various degrees, the extent depending on the glycosidase and the tissue. In some tissues there was considerable heterogeneity in the patterns of distribution for the enzymes. The degree of latency exhibited in the particles by each enzyme varied from tissue to tissue. The significance of these results with reference to the lysosome theory is discussed.