The kinetics of cholesterol oxidase synthesis by Nocardia rhodocrous
- 1 May 1976
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 18 (5), 601-621
- https://doi.org/10.1002/bit.260180502
Abstract
The production of cholesterol oxidase by 3 liter batch cultures of Nocardia rhodocrous growing on a glycerol/yeast extract medium was investigated. Cholesterol was shown to be a good inducer of the enzyme. The optimum time for cholesterol addition and the quantity to be added were determined, resulting in a 15‐fold yield increase. Cholesterol oxidase synthesis was influenced by the dissolved oxygen tension. Maximum cholesterol oxidase production was obtained at 30–40% air saturation. The effect of growth conditions on the extraction of cholesterol oxidase by Triton X‐100 was investigated. The scale‐up of the fermentation to 800 liters in a pilot‐plant fermenter is described.This publication has 16 references indexed in Scilit:
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