The Determination of the Percentages of Gγand AγChains in Human Fetal Hemoglobin by HPLC
- 1 January 1980
- journal article
- Published by Taylor & Francis in Hemoglobin
- Vol. 4 (3-4), 487-496
- https://doi.org/10.3109/03630268008996229
Abstract
A newly developed HPLC procedure (1) was used to study the percentages of G gamma and A gamma chains in the Hb F from newborn and patients with sickle cell anemia. The method gives data comparable to those obtained with a more laborious chemical procedure. However, the presence of the A gamma T chain, i.e., the A gamma chain with an Ile replaced by Thr substitution at position 75, interferes with the determination of the G gamma to A gamma ratio because the A gamma T and G gamma chains have the same chromatographic mobility.Keywords
This publication has 13 references indexed in Scilit:
- Rapid separation of the α,β,Gγ and Aγ human globin chains by reversed-phase high pressure liquid chromatographyBiochemical and Biophysical Research Communications, 1979
- Further studies of the frequency and significance of the Tgamma-chain of human fetal hemoglobin.JCI Insight, 1979
- Preliminary Experiments in the Separation of Globin Chains by high Performance Liquid ChromatographyHemoglobin, 1979
- Anomaly in the γ chain heterogeneity of the newbornNature, 1977
- An Improved Chromatographic Procedure for Quantitation of Human Fetal HemoglobinHemoglobin, 1977
- Significance of a new type of human fetal hemoglobin carrying a replacement isoleucine ? threonine at position 75 (E 19) of the ? chainHuman Genetics, 1976
- Chemical heterogeneity of fetal hemoglobin in subjects with sickle cell anemia, homozygous hb-c disease, sc-disease, and various combinations of hemoglobin variantsClinica Chimica Acta; International Journal of Clinical Chemistry, 1972
- Postnatal Changes in the Chemical Heterogeneity of Human Fetal HemoglobinPediatric Research, 1971
- Studies on the heterogeneity of hemoglobinJournal of Chromatography A, 1968
- Estimation of Small Percentages of Fœtal HæmoglobinNature, 1959