Prostacyclin-dependent coronary vasodilation in rabbit and guinea pig hearts

Abstract

Isolated hearts from rabbits or guinea pigs were perfused according to Langendorff and the coronany flow was recorded. The rabbit heart transmyocardial effluent''s content of platelet anti-aggregatory (prostacyclin-like, PCLA) activity was assayed biologically at regular intervals. Perfusion was performed with a solution gassed with 95% O2 in CO2 switching at intervals to a solution gassed with 12% O2 and 5% CO2 in N2. Perfusion with a hypoxic solution elicited reproducible increases in coronary flow. After pretreatment with indomethacin (5 .times. 10-5 M) this increase was completely abolished and in several cases it was reversed to a marked reduction in coronary flow. The transmyocardial effluent contained during perfusion with normoxic solution no detectable or only negligible amounts of PCLA. During hypoxia the efflux of PCLA into the transmyocardial effluent increased. The increase was abolished when indomethacin (5 .times. 10-5 M) was added to the solution perfusing the heart. Apparently increased coronary vascular formation of prostacyclin plays a key role in the coronary vasodilation induced by hypoxia in rabbit and guinea pig hearts.