Evidence for leukotriene C4transport mediated by an ATP-dependent glutathione S-conjugate carrier in rat heart and liver plasma membranes
- 18 December 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 259 (1), 95-98
- https://doi.org/10.1016/0014-5793(89)81503-0
Abstract
Using rat heart sarcolemma and liver plasma membrane vesicles, it has been verified that the transport of leukotriene C4 (LTC4) across membranes is an ATP-dependent process; the apparent K m for LTC4 was 150 nM (heart sarcolemma) or 250 nM (liver plasma membrane). S-(2,4-dinitrophenyl)-glutathione (DNP-SG) inhibited LTC4 uptake into the vesicles dose-dependently (I 50 = 25 (μM for both heart sarcolemma and liver plasma membrane vesicles). Mutual inhibition between LTC4 and DNP-SG in uptake into the vesicles demonstrates that transport of LTC4 is mediated by an ATP-dependent glutathione S-conjugate carrier.Keywords
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