Factors Affecting the Composition of Oligosaccharides Produced in Chitosan Hydrolysis Using Immobilized Chitosanases
- 1 January 2002
- journal article
- research article
- Published by Wiley in Biotechnology Progress
- Vol. 18 (5), 969-974
- https://doi.org/10.1021/bp020011r
Abstract
The hydrolysis reaction of chitosan using immobilized chitosanases with regard to the composition of its products and the yield of the intermediate target products, pentamer and hexamer of chitosan oligosaccharides, was investigated. Chitosanase was immobilized onto agar or agarose gel particles by the multipoint attachment method. In batch experiments, surface enzyme density, support particle size, temperature, agitator speed, and initial substrate concentration significantly affected the composition of the oligosaccharides produced. It was believed that these factors all related to the reaction rate and mass transfer rate at the surface of the support materials immobilizing the enzymes. These effects were summarized as a correlation with Damköhler number (Da), defined as the ratio of the maximum reaction rate to the maximum mass transfer rate. The result showed that the reaction conditions that give a low value of Da provide a high yield of pentamer and hexamer oligosaccharides.Keywords
This publication has 18 references indexed in Scilit:
- Immobilization and stabilization of chitosanase by multipoint attachment to agar gel supportJournal of Bioscience and Bioengineering, 2002
- Specificity of chitosanase from Bacillus pumilusBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1994
- Purification and hydrolytic action of a chitosanase from Nocardia orientalisBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1991
- Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymesEnzyme and Microbial Technology, 1988
- Growth-inhibitory effect of hexa-N-acetylchitohexaose and chitohexaose against Meth-A solid tumor.CHEMICAL & PHARMACEUTICAL BULLETIN, 1988
- Preparation of D-glucosamine oligosaccharides by the enzymatic hydrolysis of chitosan.Agricultural and Biological Chemistry, 1987
- A proposal for accurate determination of “true” fractional retention of enzyme activity on immobilizationBiotechnology & Bioengineering, 1977
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- External diffusional resistance in immobilized‐enzyme catalysisBiotechnology & Bioengineering, 1972
- The Preparation of Glucosamine Oligosaccharides. I. Separation1,2Journal of the American Chemical Society, 1957