The biological activity of pure peptides obtained by enzymic hydrolysis of insulin
- 1 May 1960
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 75 (2), 395-401
- https://doi.org/10.1042/bj0750395
Abstract
The action of carboxypeptidase on insulin and acetyl-insulin has been investigated. The results of previous workers that the removal of C-terminal alanine does not affect the biological activity c the insulin molecule, but that removal of C-terminal asparagine does so, have been confirmed. The action of trypsin on insulin has also been investigated. The peptides resulting have been purified, characterized and tested for biological activity. The peptide DHA insulin, which is insulin without the 8 amino acid residues of the carboxyl end of the phenylalanyl chain,still retains about 15% of the biological activity of the intact insulin molecule.Keywords
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