The binding of Cellulomonas fimi endoglucanase C (CenC) to cellulose and Sephadex is mediated by the N‐terminal repeats
- 1 May 1992
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 6 (9), 1243-1252
- https://doi.org/10.1111/j.1365-2958.1992.tb01563.x
Abstract
Endoglucanase C (CenC) from Celluiomonas fimi binds to cellulose and to Sephadex. The enzyme has two contiguous 150-amino-acid repeats (N1 and N2) at its N-terminus and two unrelated contiguous 100-amino-acjd repeats (C1 and C2) at its C-terminus. Polypeptides corresponding to N1, N1N2, C1, and C1C2 were produced by expression of appropriate cenC gene fragments in Escherichia coli. N1N2, but not N1 alone, binds to Sephadex; both polypeptides bind to Avicel, (a heterogeneous cellulose preparation containing both crystalline and non-crystalline components). Neither C1 nor C1C2 binds to Avicel or Sephadex. N1N2 and N1 bind to regenerated (amorphous') cellulose but not to bacterial crystalline cellulose; the cellulose-binding domain of C. fimi exoglucanase Cex binds to both of these forms of cellulose. Amino acid sequence comparison reveals that N1 and N2 are distantly related to the cellulose-binding domains of Cex and C. fimi endoglucanases A and B.Keywords
This publication has 58 references indexed in Scilit:
- Non–Hydrolytic Disruption of Cellulose Fibres by the Binding Domain of a Bacterial CellulaseBio/Technology, 1991
- The N‐terminal region of an endoglucanase from Pseudomonas fluorescens subspecies cellulosa constitutes a cellulose‐binding domain that is distinct from the catalytic centreMolecular Microbiology, 1990
- Cellulase families revealed by hydrophobic cluster analysiGene, 1989
- Glycosylation of bacterial cellulases prevents proteolytic cleavage between functional domainsFEBS Letters, 1987
- Homology between cellulase genes of Trichoderma reesei: complete nucleotide sequence of the endoglucanase I geneGene, 1986
- Structure of the gene encoding the exoglucanase of Cellulomonas fimiGene, 1986
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- SDS—PAGE strongly overestimates the molecular masses of the neurofilament proteinsFEBS Letters, 1984
- Purification and Partial Characterization of Three Extracellular from Cellulomunas sp.European Journal of Biochemistry, 1978
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970