Frequency of Allogeneic Helper T Cells Responding to Whole H-2 Differences and to an H-2K Difference Alone
- 31 August 1979
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 123 (3), 1062-1067
- https://doi.org/10.4049/jimmunol.123.3.1062
Abstract
Limiting dilution analysis was used to determine the frequency of splenic T cells that are stimulated by alloantigen to give help in a primary antibody response to SRBC. Serveral haplotype combinations were tested. A semilogarithmic plot of the fraction of nonresponding culture as a function of the number of T cells added to excess B cells gave a straight line intercepting with the origin. Thus a single cell-type was limiting, which was required to help B cells respond to SRBC. The frequency of syngeneic precursors of T helper cells specific for SRBC ranged from 1/10,000 to 1/55,000 with a mean of about 1/20,000. Allohelpers generated by whole H-2 differences gave precursor frequencies that ranged from 1/1000 to 1/7000 with a mean of about 1/2500. Thus allohelpers to whole H-2 differences were approximately 8-fold more frequent than SRBC-specific helpers. When the stimulation was limited to the H-2K difference between the mutant B6.C-H-2ba and wild-type B6, frequencies of from 1/2600 to 1/7900 allohelpers were found with a mean of about 1/5000, approximately half the frequency of allohelpers to whole H-2 differences. Thus some, but probably not all, of the magnitude of allogeneic help can be attributed to the high frequency of helper T cells that respond to a given alloantigen.This publication has 4 references indexed in Scilit:
- Alternative pathways of T lymphocyte activation.The Journal of Experimental Medicine, 1978
- T-lymphocyte response to H-2 mutants. I. Proliferation is dependent on Ly 1+2+ cells.The Journal of Experimental Medicine, 1978
- Structural Differences between Parent and Mutant H-2K Glycoproteins from Two H-2K Gene Mutants: B6.C-H-2ba (Hzl) and B6-H-2bd (M505)The Journal of Immunology, 1977
- Is There Evidence for a Non-Antigen Specific Diffusable Chemical Mediator from the Thymus-Derived Cell in the Initiation of the Immune Response?**Supported by U. S. Public Health Service Research Grant AI-08795-03 and American Cancer Society Research Grant E-395-D.Published by Elsevier ,1971