Small Molecule Ice Recrystallization Inhibitors Enable Freezing of Human Red Blood Cells with Reduced Glycerol Concentrations
Open Access
- 8 April 2015
- journal article
- research article
- Published by Springer Nature in Scientific Reports
- Vol. 5 (1), 9692
- https://doi.org/10.1038/srep09692
Abstract
In North America, red blood cells (RBCs) are cryopreserved in a clinical setting using high glycerol concentrations (40% w/v) with slow cooling rates (~1°C/min) prior to storage at −80°C, while European protocols use reduced glycerol concentrations with rapid freezing rates. After thawing and prior to transfusion, glycerol must be removed to avoid intravascular hemolysis. This is a time consuming process requiring specialized equipment. Small molecule ice recrystallization inhibitors (IRIs) such as β-PMP-Glc and β-pBrPh-Glc have the ability to prevent ice recrystallization, a process that contributes to cellular injury and decreased cell viability after cryopreservation. Herein, we report that addition of 110 mM β-PMP-Glc or 30 mM β-pBrPh-Glc to a 15% glycerol solution increases post-thaw RBC integrity by 30-50% using slow cooling rates and emphasize the potential of small molecule IRIs for the preservation of cells.Keywords
This publication has 67 references indexed in Scilit:
- Synergistic effects of liposomes, trehalose, and hydroxyethyl starch for cryopreservation of human erythrocytesBiotechnology Progress, 2012
- Inhibiting ice recrystallization and optimization of cell viability after cryopreservationGlycobiology, 2011
- Synthesis and antifreeze activity of fish antifreeze glycoproteins and their analoguesChemical Science, 2010
- Slowing the growth of ice with synthetic macromolecules: beyond antifreeze(glyco) proteinsPolymer Chemistry, 2010
- Will the original glucose transporter isoform please stand up!American Journal of Physiology-Endocrinology and Metabolism, 2009
- A multicenter study of in vitro and in vivo values in human RBCs frozen with 40‐percent (wt/vol) glycerol and stored after deglycerolization for 15 days at 4°C in AS‐3: assessment of RBC processing in the ACP 215Transfusion, 2001
- Mammalian passive glucose transporters: members of an ubiquitous family of active and passive transport proteinsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1993
- Interactions of sugars with membranesBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1988
- Het mechanism of the protective action of glycerol against haemolysis by freezing and thawingBiochimica et Biophysica Acta, 1953
- The Standardization of Hemoglobin MeasurementThe American Journal of the Medical Sciences, 1948