Synergistic effects of liposomes, trehalose, and hydroxyethyl starch for cryopreservation of human erythrocytes
- 9 February 2012
- journal article
- research article
- Published by Wiley in Biotechnology Progress
- Vol. 28 (2), 364-371
- https://doi.org/10.1002/btpr.1519
Abstract
Red blood cells (RBCs) can be cryopreserved using glycerol as a cryoprotective agent, but one of the main disadvantages is the time‐consuming deglycerolization step. Novel cryopreservation strategies for RBCs using nontoxic cryoprotective agents are urgently needed. The effect of DMPC, DOPC, and DPPC liposomes on survival of RBCs cryopreserved with trehalose and HES has been evaluated. DMPC caused hemolysis before freezing and affected RBC deformability parameters. DMPC treated RBCs displayed a strong increase in trehalose uptake compared to control cells, whereas DOPC treated liposomes only displayed a slight increase in trehalose uptake. High intracellular trehalose contents were observed after cryopreservation. The recovery of cells incubated with trehalose and liposomes, frozen in HES ranged between 92.6 and 97.4% immediately after freezing. Recovery values of RBCs frozen in HES, however, decreased to 66.5% after 96 h at 4°C compared to 77.5% for DOPC treated RBCs. The recovery of RBCs incubated and frozen in trehalose medium was 77.8%. After 96 hours post‐thaw storage recovery of these cells was 81.6%. DOPC and DPPC treated RBCs displayed higher recovery rates (up to 89.7%) after cryopreservation in trehalose compared to control RBCs. Highest survival rates were obtained using a combination of trehalose and HES: 97.8% directly after thawing and 81.8% 96‐h post‐thaw. DOPC liposomes, trehalose and HES protect RBCs during cryopreservation in a synergistic manner. The advantage is that the protective compounds do not need to be removed before transfusion. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012Keywords
This publication has 34 references indexed in Scilit:
- Membrane hydraulic permeability changes during cooling of mammalian cellsBiochimica et Biophysica Acta (BBA) - Biomembranes, 2011
- Liposomes alter thermal phase behavior and composition of red blood cell membranesBiochimica et Biophysica Acta (BBA) - Biomembranes, 2011
- Membrane Stability during Biopreservation of Blood CellsTransfusion Medicine and Hemotherapy, 2011
- Investigating Interactions of Trehalose-Containing Liposomes with Human Red Blood CellsCell Preservation Technology, 2008
- Clinical consequences of red cell storage in the critically illTransfusion, 2006
- Recommendations for reference method for haemoglobinometry in human blood (ICSH standard 1995) and specifications for international haemiglobinocyanide standard (4th edition).Journal of Clinical Pathology, 1996
- A Simplified Procedure for Deglycerolizing Red Blood Cells Frozen in a High Glycerol ConcentrationTransfusion, 1977
- Hydroxyethyl Starch: Extracellular Cryophylactic Agent for ErythrocytesScience, 1967
- Effect of Lactose, Dextran and Albumin on Recovery and Survival of Frozen Red CellsTransfusion, 1965
- PREVENTION OF HqMOLYSIS DURING FREEZING AND THAWING OF RED BLOOD-CELLSThe Lancet, 1950