Protein Aggregation in Conventional and Ultra High-Temparature Heated Skimmilk
Open Access
- 1 August 1969
- journal article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 52 (8), 1174-1180
- https://doi.org/10.3168/jds.s0022-0302(69)86720-2
Abstract
Ultracentrifugate supernatant fractions from raw and heated skimmilk were examined by Sephadex G-100 gel-filtration and zonal electorphoresis to investigate the protein aggregation produced by heating skimmilk at 90 C for 10 or 30 min, and at temperatures to 149 C for as long as 16 sec. Heating at 90 C caused preferential formation of intermediate size ≥75 to 100 S ≤425 to 500 S protein particles; whereas, the ultra high-temperature heat treatments favored formation of larger size ≥425 to 500 S particles. Ultra high-temperature heat treatments produced only slight alteration of Sephadex gel-filtration patterns for the smallest size ≤75 to 100 S protein components; whereas, all of the ≤75 to 100 S protein components in 90 C heated skimmilk were sufficiently aggregated as to be totally excluded from Sephadex G-100. Polyacrylamide gel electrophoresis data indicated that the rate and extent of heat-induced protein aggregation to particle sizes in excess of about 75 to 100 S closely parallel those for whey protein denaturation. The findings support the concept that ultra high-temperature heat treatments of 146 to 150 C may produce small amounts of nonsedimenting casein components by disaggregation of casein micelles.Keywords
This publication has 7 references indexed in Scilit:
- Effect of Calcium, N-Ethylmaleimide and Casein upon Heat-Induced Whey Protein AggregationJournal of Dairy Science, 1968
- Relation of Heat-induced Changes in Protein-salt Constituents to Astringency in Milk SystemsJournal of Dairy Science, 1967
- Effects of High-Heat Treatment on Stability of Calcium Caseinate Aggregates in MilkJournal of Dairy Science, 1967
- Denaturation of Whey Proteins in Milk Heated at High Temperatures for Short TimesJournal of Dairy Science, 1966
- Factors Affecting the Heat Stability of MilkJournal of Dairy Science, 1962
- Photomicrographs of Milk Protein ParticlesJournal of Dairy Science, 1961
- The Reducing Capacity of Milk as Measured by an Iodimetric TitrationJournal of Dairy Science, 1950