No effect of extremely low‐frequency magnetic field observed on cell growth or initial response of cell proliferation in human cancer cell lines
- 17 June 2002
- journal article
- research article
- Published by Wiley in Bioelectromagnetics
- Vol. 23 (5), 355-368
- https://doi.org/10.1002/bem.10006
Abstract
An effect on the tumor promotion process, as represented by accelerated cell growth, has been indicated as one example of areas that demonstrate the possibility of biological effects of extremely-low frequency magnetic fields. We, therefore, exposed the five cell lines (HL-60, K-562, MCF-7, A-375, and H4) derived from human tumors to a magnetic field for 3 days to investigate the effects on cell growth. Prior to exposure or sham exposure, the cells were precultured for 2 days in low serum conditions. The number of growing cells was counted in a blind manner. To investigate the effect on the initial response of cell proliferation, two cell lines were synchronized in G1 phase by serum starvation and then exposed to a magnetic field for 18 h (H4 cells) or 24 h (MCF-7 cells), both with and without serum stimulation. The rate of DNA synthesis, taken as a measure of the cell proliferation, was determined by following the incorporation of [3H]-thymidine into the DNA. Three different magnetic field polarizations at both 50 and 60 Hz were used: linearly polarized (vertical); circularly polarized; and an elliptically polarized field. Magnetic field flux densities were set at 500, 100, 20 and 2 μT (rms) for the vertical field and at 500 μT (rms) for the rotating fields. No effect of magnetic field exposure was observed on either cell growth or the initial response of cell proliferation. Bioelectromagnetics 23:355–368, 2002.Keywords
This publication has 30 references indexed in Scilit:
- In vitro and in vivo effects of a cyclic peptide with affinity for the ανβ3 integrin in human melanoma cellsEuropean Journal Of Cancer, 2000
- Cytological effects of 50 Hz electromagnetic fields on human lymphocytes in vitroMutation Research Letters, 1995
- Changes in cell proliferation due to environmental non-ionizing radiation 1. ELF electromagnetic fieldsBioelectrochemistry and Bioenergetics, 1995
- Specific region of the c-myc promoter is responsive to electric and magnetic fieldsJournal of Cellular Biochemistry, 1994
- Transcription and translation in cells exposed to extremely low frequency electromagnetic fieldsBioelectrochemistry and Bioenergetics, 1991
- Reproductive integrity of mammalian cells exposed to power frequency electromagnetic fieldsEnvironmental and Molecular Mutagenesis, 1991
- Nonthermal 60 Hz sinusoidal magnetic‐field exposure enhances 45Ca2+ uptake in rat thymocytes: dependence on mitogen activationFEBS Letters, 1990
- Effects of 50-Hertz electromagnetic fields on proliferation and on chromosomal alterations in human peripheral lymphocytes untreated or pretreated with chemical mutagensMutation Research, 1989
- Use of in Vivo/In Vitro Unscheduled Dna Synthesis for Identification of Organ-Specific CarcinogensCRC Critical Reviews in Toxicology, 1987
- Continuous growth and differentiation of human myeloid leukaemic cells in suspension cultureNature, 1977