Isolation of functional human trophoblast cells and their partial characterization in primary cell culture
- 1 July 1978
- journal article
- research article
- Published by Springer Nature in In Vitro Cellular & Developmental Biology - Plant
- Vol. 14 (7), 631-638
- https://doi.org/10.1007/bf02617924
Abstract
Human trophoblast isolation and cell culture procedures were examined to identify variables that enhance secretion of chorionic gonadotropin (HCG) in primary culture. Brief exposure of unminced first-trimester placental specimens to a solution of trypsin-EDTA-DNAse, and isolation of the dispersed cells after Ficoll-hypaque centrifugation yielded primary cultures that were high in HCG secretion and content of epithelial-like cells. The gradual decline in HCG level with time in monolayer culture in these presumptive trophoblast cells was retarded by treatment with theophylline and cyclic adenosine monophosphate. Exposure to methotrexate (MTX) did not increase HCG secretion in normal trophoblast cells, in contrast to a 5-fold stimulation by MTX in the JAR line of choriocarcinoma cells. Clusters of polygonal cells in primary culture progressively lost their capacity to secrete HCG and their epithelial-like morphology. However, they could be maintained as cell strains through approximately 15 passages over a period of 13 to 16 weeks.This publication has 19 references indexed in Scilit:
- Effect of cyclic 3′:5′-AMP derivatives, prostaglandins and related agents on human chorionic gonadotropin secretion in human malignant trophoblast in cultureIn Vitro Cellular & Developmental Biology - Plant, 1977
- The biosynthesis of progesterone by cultured mouse midgestation trophoblast cellsDevelopmental Biology, 1975
- Early Stimulation of Human Chorionic Gonadotropin Secretion by Dibutyryl Cyclic AMP and Theophylline in Human Malignant Trophoblast Cells in vitro: Inhibition by Actinomycin D, α-Amanitin, and CordycepinGynecologic and Obstetric Investigation, 1975
- VIABILITY OF HUMAN TROPHOBLAST IN VITROBJOG: An International Journal of Obstetrics and Gynaecology, 1973
- Interpretation of the Multinucleated Giant Cell in Human Trophoblast CulturesGynecologic and Obstetric Investigation, 1973
- Immunofluorescent Localization of Chorionic Gonadotrophin in Monolayer Cultures of Human Trophoblast CellsNature, 1970
- Morphology and enzyme histochemistry of cells derived from placental villi in tissue cultureThe Journal of Pathology, 1970
- Clinical PhysicsScandinavian Journal of Clinical and Laboratory Investigation, 1968
- On the Identification of Trypsin-released Human Placental Cells and Their Separation MethodThe Tohoku Journal of Experimental Medicine, 1964
- A Method for Obtaining Monolayer Cultures of Human Fetal Cells from Term Placentas.Experimental Biology and Medicine, 1961