New Sites of Human S-100 Immunoreactivity Detected with Monoclonal Antibodies
Open Access
- 1 February 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 85 (2), 160-168
- https://doi.org/10.1093/ajcp/85.2.160
Abstract
In a previous paper from this laboratory, the production of monoclonal antibodies recognizing antigenic determinants common to the alpha and beta chains of bovine brain S-100 protein was reported. In the present study, the immunohistochemical labeling patterns of these monoclonal antibodies against a wide range of normal and pathologic human tissues are described, and these results are compared with those obtained using polyclonal anti-S-100 antiserum. Although many of the reactions of the monoclonal antibodies were very similar to those of the polyclonal antiserum (and the previously reported sites of S-100 immunoreactivity) several additional cell types (e.g., thyroid follicular cells, biliary epithelium, pancreatic cells, renal tubules) were labeled by one or both of the monoclonal antibodies. Blocking experiments prove that immunohistochemical differences obtained with monoclonal and polyclonal antibodies are at least partly caused by differences in the repertoire of antigenic determinants on S-100 protein that are recognized by the two species (i.e., mouse and rabbit, respectively). These results indicate that S-100 may be more widespread in human tissues than previously thought, and that its value as a marker of the histogenetic origin of human tumors should be reappraised. It is suggested that the marked variability observed in the reactivity of different tissues in the present study may indicate that S-100 is a heterogenous group of molecules, and its expression may be related to the functional activity of cells.Keywords
This publication has 15 references indexed in Scilit:
- Malignant histiocytosis and Letterer-Siwe disease neoplasms of T-zone histiocyte with s100 proteinCancer, 1983
- Role of Antibody to S100 Protein in Diagnostic PathologyAmerican Journal of Clinical Pathology, 1983
- Purification and characterization of adipose tissue S-100b protein.Journal of Biological Chemistry, 1983
- An immunoperoxidase study of S-100 protein distribution in normal and neoplastic tissuesThe American Journal of Surgical Pathology, 1982
- Immunohistochemical demonstration of S 100 protein in malignant melanoma and pigmented nevus, and its diagnostic applicationCancer, 1982
- S-100 PROTEIN IN SOFT-TISSUE TUMORS DERIVED FROM SCHWANN-CELLS AND MELANOCYTES1982
- S-100 PROTEIN IN LANGERHANS CELLS, INTERDIGITATING RETICULUM CELLS AND HISTIOCYTOSIS X-CELLS1982
- LOCALIZATION OF S-100 PROTEIN AND GLIAL FIBRILLARY ACIDIC PROTEIN-RELATED ANTIGEN IN PLEOMORPHIC ADENOMA OF THE SALIVARY-GLANDS1982
- Immunohistochemical and immunoelectron microscopic localization of S-100 protein in the interdigitating reticulum cells of the human lymph nodeVirchows Archiv B Cell Pathology Including Molecular Pathology, 1981
- S100 protein in folliculostellate cells of the rat pituitary anterior lobeBrain Research, 1980