Distribution and Fractionation of the Monkey Anti-Anemia Factor

Abstract

Further studies were carried out on the distribution and fractionation of the monkey anti-anemia factor. Whey solids, yellow corn, raw potato and raw carrot are poor sources of the factor. Higher levels of folic acid and rather high levels of rutin and hesperidin, L-lysine and DL-tryptophane, and pernicious anemia concentrates failed to show any activity for the monkey. Approximately 4 gm of fresh liver are needed by the monkey per day. An ethanol extract of fresh liver was active as well as a methanol and acetone extract of liver. A norite eluate prepared from the alcohol extract also proved active when fed at a higher level. A tenfold concentration was observed by the norite elution method. Atttempts were made to shorten the assay by feeding sulfasuxidine in the ration, lowering the protein intake, and by feeding fat free rations.